It’s a match that could prove a test of Italy’s strength and Italy’s form.
The hosts have a tough task ahead of them as they have only won two of their last nine meetings, including a 4-1 defeat at the hands of Spain last week.
They will be without captain Alessandro Nesta, who is expected to be unavailable for Saturday’s clash, but are expected to have their best defence to counter the attack.
The Italians have only beaten Spain once this season and will be hoping to maintain their form on the road.
The Spain squad that has made the most progress in this World Cup has been made up of youngsters, but will be in good form with Nesta and Fabio Cannavaro in contention.
This will be the first meeting between the two teams in the tournament, with Spain coming away with a 1-0 win at the Stadio Olimpico in April 2015.
They are also unbeaten in their last eight home matches, including their opening three fixtures in this tournament.
Mario Balotelli and Paul Pogba will have to do more than just their usual self-assurance against a Spanish defence that has conceded nine goals in their five matches this tournament, but they will also need to be better than last season, when they lost to Colombia 2-1 at the Maracana in the final round.
They have only scored one goal in their past seven home games against Spain, but should be able to get back on track as they are unbeaten in four of their six group games.
Spain are in a bit of a precarious position with both Lionel Messi and Sergio Aguero injured and will not be able have any real effect on Italy’s chances.
Their attacking players, notably Messi, are also likely to be out for the rest of the season due to injury.